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KMID : 0545120130230010131
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 1 p.131 ~ p.135
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Synthesis of NBD-Labeled DOTAP Analog to Track Intracellular Delivery of Liposome
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Doh Kyung-Oh
Kim Bieong-Kil
Lee Tae-Jin
Park Jong-Won
Seu Young-Bae
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Abstract
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A DOTAP analog labeled by NBD on the head group (DTNBD) was designed and synthesized to label DOTAP liposome. The structure was confirmed by 1H NMR and FAB-MS, and the fluorescence of the newly synthesized DT-NBD was observed by fluorescent microscopy. The transfection efficiency of DOTAP liposome containing DT-NBD was comparable to commonly used NBD PE in COS7 and MCF7 cells. Furthermore, the level of cellular uptake and fluorescent intensity of fluorescent liposome containing DT-NBD was higher than NBD PE. Therefore, the novel NBD-labeled DOTAP analog seems to be effectively used for investigation of the cellular interaction and transfection mechanism of DOTAP liposome.
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KEYWORD
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DOTAP, fluorescent lipid, cationic liposome, transfection
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